Purpose
This course deals with the DNA isolation and transfer to plants and animals and production of transgenic animals and plantsInstructional Objectives
1. To impart knowledge on production of transgenic animals and plants2. To make the students to understand the concepts and applications
| S.No | Course Title | Downloads | ||
| UNIT I - Plant Transformation | ||||
| 1. | Agro bacterium mediated plant transformation |  (Size:109.0 KB) |
 (Size:7658.0 KB) |
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| 2. | Ti plasmids derived vector system | (Size:91.0 KB) |
(Size:1175.0 KB) |
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| 3. | Transfer of genes to plants | (Size:155.0 KB) |
(Size:2827.0 KB) |
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| 4. | Reporter genes | (Size:60.0 KB) |
(Size:1002.0 KB) |
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| 5. | Marker genes and production of marker free transgenic plants | (Size:119.0 KB) |
(Size:299.0 KB) |
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| 6. | Manipulation of gene expression and secretion of foreign protein | (Size:290.0 KB) |
(Size:395.0 KB) |
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| 7. | Plant tissue culture and applications | (Size:117.0 KB) |
(Size:1547.0 KB) |
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| UNIT II - Genetic Engineering of Plants and Animals | ||||
| 8. | Insect resistance plants | (Size:595.0 KB) |
(Size:2724.0 KB) |
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| 9. | Virus resistance plants | (Size:2573.0 KB) |
(Size:1505.0 KB) |
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| 10. | Herbicide resistance plants | (Size:1766.0 KB) |
(Size:2582.0 KB) |
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| 11. | Stress and senescence tolerant plants | (Size:1488.0 KB) |
(Size:1199.0 KB) |
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| 12. | Genetic manipulation of flower pigmentation | (Size:1179.0 KB) |
(Size:263.0 KB) |
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| 13. | Modification of plant nutrient content | (Size:1114.0 KB) |
(Size:925.0 KB) |
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| 14. | Modification of plant food taste and appearance | (Size:685.0 KB) |
(Size:653.0 KB) |
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| UNIT III - Genetic Engineering of Animals | ||||
| 15. | Basics of transgenic animals | (Size:40.0 KB) |
(Size:527.0 KB) |
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| 16. | Methods for producing transgenic animals | (Size:53.0 KB) |
(Size:504.0 KB) |
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| 17. | Retroviral vector method | (Size:141.0 KB) |
(Size:335.0 KB) |
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| 18. | DNA microinjection method | (Size:299.0 KB) |
(Size:653.0 KB) |
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| 19. | Engineered embryonic stem cell method | (Size:985.0 KB) |
(Size:1085.0 KB) |
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| 20. | Transgenesis with high capacity vectors | (Size:176.0 KB) |
(Size:488.0 KB) |
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| UNIT IV - Cloning Livestock by Nuclear Transfer | ||||
| 21. | Cloning by nuclear transfer | (Size:42.0 KB) |
(Size:173.0 KB) |
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| 22. | Cloning Dolly | (Size:39.0 KB) |
(Size:813.0 KB) |
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| 23. | Transgenic cattle | (Size:181.0 KB) |
(Size:1451.0 KB) |
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| 24. | Transgenic sheep, goat and pigs | (Size:126.0 KB) |
(Size:777.0 KB) |
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| 25. | Transgenic birds | (Size:70.0 KB) |
(Size:74.0 KB) |
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| 26. | Transgenic fish | (Size:25.0 KB) |
(Size:1415.0 KB) |
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| UNIT V - Plants and Animals as Bioreactors | ||||
| 27. | Plants as bioreactors | (Size:930.0 KB) |
(Size:6395.0 KB) |
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| 28. | Antibody production | (Size:22.0 KB) |
(Size:2876.0 KB) |
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| 29. | Polymers | (Size:160.0 KB) |
(Size:332.0 KB) |
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| 30. | Edible vaccines | (Size:617.0 KB) |
(Size:1614.0 KB) |
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| 31. | Animals as bioreactors-therapeutic proteins | (Size:277.0 KB) |
(Size:265.0 KB) |
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| 32. | Enzymes and vaccines | (Size:30.0 KB) |
(Size:10.0 KB) |
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Reference
- Molecular Biotechnology by R.Click and J.Pasternek
- Animal transgenesis and cloning by Louis-Marie Houdebeine(E-BOOK)
