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Dr. Saswat Sourav Mohapatra

M.Sc., Ph.D

Research Assistant Professor

I started working as a Research Assistant Professor at the SRM University from August 2016. I am affiliated to the SRM Research Institute and the Department of Genetic Engineering. My current research work is focused in the area of molecular microbiology, and my long-term research interests include Bacterial Cell Cycle Regulation and Signal Transduction Mechanisms.

Education-

  • PhD (Life Science)- Institute of Life Sciences, Utkal University, Bhubaneswar, India (2010)
  • MSc (Microbiology)- Orissa University of Agriculture & Technology, Bhubaneswar, India (2003)

Research Experience-

Postdoctoral Research:

  • Sep, 2009 – Jan, 2012- Department of Microbiology, Molecular Genetics & Immunology, University of Kansas Medical Center, Kansas City, USA. Project- Modulation of virulence gene expression in the pathogen Streptococcus mutans, mediated by various two-component signal transduction systems. Advisor - Dr. Indranil Biswas
  • Mar, 2012 – Sep, 2014- Institut de Recherche Interdisciplinaire, CNRS USR 3078, Villeneuve d’Ascq, France. Project- Cell cycle regulation in the bacterial model system Caulobacter crescentus. Advisor - Dr. Emanuele Biondi
  • Dec, 2015 –Aug, 2016- Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, India. Project- Role of R-loops (RNA-DNA hybrids) in generation of transcription-replication conflicts in Escherichia coli. Advisor – Dr. J. Gowrishankar

Doctoral Research:

  • Feb, 2004 – Aug, 2009- Infectious Disease Biology, Institute of Life Sciences, Utkal University, Bhubaneswar, India
  • Title of the PhD thesis- Heterogeneity among Vibrio cholerae O1 environmental strains in relation to SXT-ICEs and CTX genetic element. Advisor- Dr. Durg V. Singh

 

My research interests include Bacterial Cell Cycle Regulation and Signal Transduction mechanisms.

1. Bacterial Cell Cycle Regulation-

Epigenetic mechanisms regulating various physiological activities in the prokaryotic cells are increasingly being appreciated. Methylation of specific bases of the DNA molecule by methyltransferases is the most common epigenetic modification observed in the bacterial cells. This modification of nucleotides adds another level of regulation at the transcription; furthermore, it has a fundamental role in the cell physiological processes such as DNA replication, DNA mismatch repair, and virulence mechanisms in many pathogens. Among the prokaryotic DNA methyltransferases, Dam expressed among the Gammaproteobacteria is the most intensively studied. In contrast to Dam, another DNA methyltransferase CcrM (Cell Cycle Regulated Methylase) has been described in the Alphaproteobacteria, where it plays an important role in the cell cycle regulation of Caulobacter crescentus and Agrobacterium tumefaciens, in the infection process of Brucella abortus, and in the symbiotic mechanism involving Sinorhizobium meliloti etc. Both Dam and CcrM methylate the adenine residue in the respective recognition motifs 5’-GATC-3’ and 5’-GAnTC-3’. Among the several functional differences between Dam and CcrM, the most prominent is the role of the later in cell cycle regulation (Fioravanti et al., 2013; Mohapatra et al., 2014). My interest in this area is to understand how DNA methylation plays a role in coordinating several physiological processes in a cell cycle dependent manner using the Alphaproteobacteria model Caulobacter crescentus.

2. Bacterial Signal Transduction Mechanisms-

Bacteria sense and respond to their environment by several signaling mechanisms, the most important of which is two-component systems (TCS). The canonical TCS consists of a sensor kinase, also known as histidine kinase, and a response regulator. The sensor kinase senses various external signals and in turn gets auto-phosphorylated and then transmits the signal to the response regulator in a phosphorylation dependent pathway. The phosphorylated response regulator generally functions as a transcription factor and modulates the expression of several genes. My interest in this area is to understand the role of two-component systems in developing antibiotic resistance in bacterial pathogens. The long-term goal is to develop possible intervention methods that target this signal transduction process.

M. Tech.

  • Ms. Infencia Xavier

Ph.D

  • To be updated

Google Scholar Profile-

https://scholar.google.co.in/citations?user=1mz_OBEAAAAJ&hl=en&oi=ao

Peer-reviewed articles-

  1. Saswat S. Mohapatra, Chinmay K. Mantri, Tilothama Bhotra & Durg V. Singh. Characteristics of Vibrio cholerae O1 isolated from water of the River Ganga, Varanasi, India. Indian Journal of Medical Microbiology 2015; 33(4): 507-515
  2. Manoharan Shankar, Saswat S. Mohapatra,Saswati Biswas & Indranil Biswas. Gene regulation by the LiaSR two-component system in Streptococcus mutans. PLoS ONE 2015; 10(5): e0128083
  3. Saswat S. Mohapatra,Antonella Fioravanti & Emanuele G. Biondi. DNA Methylation in Caulobacter and other Alphaproteobacteria during cell cycle progression. Trends in Microbiology2014; 22(9): 528-535.
  4. Francesco Pini, Benjamin Frage, Lorenzo Ferri, Nicole J. De Nisco, Saswat S. Mohapatra, Lucilla Taddei, Antonella Fioravanti, Frederique Dewitte, Marco Galardini, Matteo Brilli, Vincent Villeret, Marco Bazzicalupo, Alessio Mengoni, Graham C. Walker, Anke Becker & Emanuele G. Biondi. The essential DivJ, CbrA kinase and PleC system controls DivK phosphorylation and symbiosis in Sinorhizobium meliloti. Molecular Microbiology 2013; 90: 54-71.
  5. Antonella Fioravanti, Coralie Fumeaux, Saswat S. Mohapatra, Coralie Bompard, Matteo Brilli, Antonio Frandi, Vincent Castric, Vincent Villeret, Patrick Viollier & Emanuele G. Biondi. DNA binding of the cell cycle transcriptional regulator GcrA depends on N6-adenosine methylation in Caulobacter crescentus and other Alphaproteobacteria. PLoS Genetics 2013; 9(5): e1003541.
  6. Indranil Biswas &Saswat S. Mohapatra. CovR alleviates transcriptional silencing by a nucleoid-associated protein HLP in Streptococcus mutans. Journal of Bacteriology 2012; 194: 2050-2061.
  7. Saswat S. Mohapatra, Chinmay K. Mantri, MHU Turabe Fazil & Durg V. Singh. Vibrio cholerae O1 biotype El Tor strains isolated in 1992 from Varanasi, India harboured El Tor CTXΦ and classical ctxB on the chromosome-I and classical CTXΦ and classical ctxB on the chromosome-II. Environmental Microbiology Reports 2011; 3: 783-790.
  8. Partho Chattoraj*, Saswat S. Mohapatra*, JL Uma Maheshwar Rao* & Indranil Biswas. Regulation of transcription by SMU.1349, a TetR-family regulator, in Streptococcus mutans. Journal of Bacteriology 2011; 193: 6605-6613. (*Equal Contribution)
  9. Alexander Dmitriev, Saswat S. Mohapatra, Patrick Chong, Melody Neely, Saswati Biswas & Indranil Biswas: CovR-controlled global regulation of gene expression in Streptococcus mutans. PLoS ONE 2011; 6(5): e20127.
  10. Chinmay K. Mantri, Saswat S. Mohapatra& Durg V. Singh: Effect of storage and sodium chloride on excision of CTXΦ or pre-CTXΦ and CTXΦ from Vibrio cholerae O139 strains. Infection, Genetics and Evolution2010; 10: 925-930.
  11. Saswat S. Mohapatra, Chinmay K. Mantri, Harapriya Mohapatra, Rita R. Colwell & Durg V. Singh: Analysis of clonally related environmental Vibrio cholerae O1 El Tor isolated before 1992 from Varanasi, India reveals origin of SXT-ICEs belonging to O139 and O1 serogroups. Environmental Microbiology Reports 2010; 2: 50-57.
  12. Chinmay K. Mantri, Saswat S. Mohapatra Rita R. Colwell & Durg V. Singh: Sequence analysis of Vibrio cholerae orfU and zot from pre-CTXΦ and CTXΦ reveals multiple origins of pre-CTXΦ and CTXΦ. Environmental Microbiology Reports 2010; 2: 67-75.
  13. Saswat S. Mohapatra, Dhanya Ramachandran, Chinmay K. Mantri, Rita R. Colwell & Durg V. Singh: Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns. Research in Microbiology 2009; 160: 57-62.
  14. Harapriya Mohapatra, Saswat S. Mohapatra, Chinmay K. Mantri, Rita R. Colwell & Durg V. Singh: Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes. Environmental Microbiology2008; 10: 866–873.
  15. Saswat S. Mohapatra, Dhanya Ramachandran, Chinmay K. Mantri & Durg V. Singh: Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, southern India. Journal of Medical Microbiology2007; 56: 260–265.
  16. Chinmay K. Mantri, Saswat S. Mohapatra, Thandavarayan Ramamurthy, Raikamal Ghosh, Rita R. Colwell & Durg V. Singh.  Septaplex PCR assay for rapid identification of Vibrio cholerae including detection of virulence and intSXT genes. FEMS Microbiology Letters 2006; 265: 208–214.

Book Chapters-

  1. Saswat S. Mohapatra & Emanuele G. Biondi. DNA Methylation in Prokaryotes: Regulation and Function. In Cellular Ecophysiology of Microbe, Handbook of Hydrocarbon and Lipid Microbiology. 2017.  Ed. T. Krell. Springer Internationational Publication.
  2. Saswat S. Mohapatra & Indranil Biswas. Microbial community interactions of the cariogenic organism Streptococcus mutans. InOral Microbial Ecology Current Research and New Perspectives, pp 133-152, 2013, eds. Nicholas S. Jakubovics & Robert J. Palmer Jr. Caister Academic Press, Norfolk, United Kingdom.
  3. Harapriya Mohapatra, Saswat S. Mohapatra, Chinmay K. Mantri & Durg V. Singh: Horizontal gene transfer and ICEs: a perspective with reference to SXT ICEs in Vibrio cholerae. In New Horizons in Biotechnology, pp388-403, 2008, eds. Ashok Pandey, Christian Larroche, Carlos Ricardo Soccol & C-G Dussap, Asiatech Publishers, Inc, Delhi.

B. Tech. Courses-

  • Microbial Genetics
  • Immunology
  • Bioengineering Instrumentation
  • Ethical Issues and Intellectual Property Rights
  • Microbial Genetics Lab
  • Molecular Techniques Lab

M. Tech. Courses-

  • Pharmacogenetics 

Opportunities:

  • Will be updated soon

Contact:
Dr. Saswat S. Mohapatra
Research Assistant Professor
SRM Research Institute
Department of Genetic Engineering
School of Bioengineering
SRM University, Kattankulathur- 603203
Tamil Nadu, India
Email: saswatsourav.h@ktr.srmuniv.ac.in
Phone- +91-44-2741-7853